FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13888385
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC633380.4560501454992787No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC372750.26838973717966486No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC268480.19331261338161349No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA57900.064.6452944
GCGACAT58550.064.00835
CTGCGAC64650.057.752183
GCTGCGA71200.053.039752
CGACATC80200.046.668856
GGCTGCG94150.040.7682951
CTAGGCG212100.033.187777
ACCCGAC26750.032.4968763
TAGGCGG217350.032.342438
TGGCTAG224400.031.772194
GCTAGGC227700.031.1855476
GGCTAGG236850.030.2221975
GACATCT133350.028.316837
GGCTGGC254950.028.0795231
CTGGCTA262200.027.4831943
ACATCTG142300.026.4023238
ACTCGGG104500.026.275021
GCTGGCT273100.025.9862252
AGGCGGG273750.025.904569
TTGATCG72900.025.89952922-23
Produced by FastQC (version 0.11.5)