FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17856362
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC593520.332385734563401No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT381180.21347013462204673No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT355960.19934631701575048No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC345430.19344925914920408No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC248840.13935649378076004No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG231020.12937685739121998No Hit
GAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTAT183680.10286529809375505No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA49350.047.3583374
GCGACAT49300.047.2085085
CTGCGAC59050.040.0703243
CGACATC59000.039.6091166
GCTGCGA64750.036.8379632
CTAGGCG198300.031.0905177
TAGGCGG207750.029.630988
TGGCTAG214250.029.554794
GCTAGGC219450.028.2027136
GGCTGCG92600.026.9444921
GGCTAGG238050.026.5172165
ACTCGGG301750.026.3493771
CTCGGGA315550.025.1767542
CTGGCTA264500.024.6816653
ACGGACC51400.024.675544
GGCTGGC255250.024.3908671
TCGGGAG331650.024.068153
GCGCGGT95850.023.1423156
GCTGGCT274500.023.0391982
TCGCACG41450.022.0031542-43
Produced by FastQC (version 0.11.5)