FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17856362
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG400910.2245194177851009No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG215110.12046686777519408No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC157950.057.6268778
TCCGACC158100.057.5121089
TTTCCGA177800.051.30037
GTTTCCG159450.048.5330126
CGTTTCC164850.044.4124035
CCGTTTC170750.042.2143824
TCCGTTT163450.040.269583
CTCCGTT125800.040.081112
GTTCCGT38100.039.6654131
GCTCCGT121350.038.2617341
GTTCACC458900.036.8331037
GTCCGGT23300.035.8972932
CGGGGAT157300.035.1840978
TCACCCC481400.034.983199
TTCACCC488700.034.5967188
TGTCGGG34550.033.140545
ATAGCGC270250.033.13947394-95
TCGGGGA167300.033.1095357
ACCGGTT22550.031.8231123
CCCGTTA17800.030.9706463
Produced by FastQC (version 0.11.5)