FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18721285
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC526290.28111852364835No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC308650.16486581984089232No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC299590.16002640844365118No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA55800.056.180384
GCGACAT59450.052.5711945
CTGCGAC67650.046.5523453
GCTGCGA74250.042.6702542
CGACATC86300.036.270076
GGCTGCG103700.031.6988891
CTAGGCG205650.028.5468227
TAGGCGG213700.027.5381498
TGGCTAG231950.026.2728544
GCTAGGC225950.026.2136756
GGCTAGG238200.025.3640825
ACTCGGG133200.022.431751
GGCTGGC281550.021.8995841
ACCCGAC29350.021.8484943
GCGCGGT43850.021.5554586
CTGGCTA288950.021.0418113
TTGATCG76850.020.79787822-23
AGGCGGG287450.020.7702149
CTCGGGA149350.020.2595622
GCTGGCT306500.019.8679642
Produced by FastQC (version 0.11.5)