FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18721285
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG261320.13958443557693823No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC59000.041.2245459
TTCCGAC60600.040.6064538
CGGGGAT178900.039.777678
TGTCGGG42850.038.5864455
TCGGGGA187500.038.1301467
CCCCGTT50700.032.153162
CTGTCGG50350.031.709654
CCCCCGT43650.031.6844581
CCGTTTC86300.031.5497154
GTTCCGT29400.031.1994921
GTCGGGG57450.030.5942846
CTATCGG99800.030.2815594
GTCCCGT32250.030.210761
CCGATCG122350.030.12384286-87
ATCGGGG136200.030.0299556
ATAGCGC114950.030.01879394-95
CGTTTCC79400.029.9196055
TTTCCGA82850.029.8156367
TATCGGG108650.029.6050175
TCTATCG98950.029.4912783
Produced by FastQC (version 0.11.5)