FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8949767
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT657280.7344101807343141No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307360.34342793505127006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297510.33242206193747836No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97730.1091983735442498No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107800.081.229841
GTATCAA305250.071.297161
ATCAACG399600.054.7291033
TCAACGC407300.053.723384
CAACGCA408050.053.5802765
AACGCAG427050.051.217346
TATCAAC448350.048.8997042
ACGCAGA464750.047.061867
CGCAGAG467250.046.796548
GCAGAGT508950.043.067559
TCTGTCG30450.034.7807048
AGAGTAC536750.031.4369110-11
GTCTCGC28350.030.8663961
CTCGCTC28450.029.9197853
CGCTCTG29300.028.6450045
CAGAGTA539500.028.50301410-11
TCTCGCT31650.027.0838552
CTGTCGC40750.027.0115689
AGTACGG198100.026.68570112-13
AGTACTT446250.026.21265412-13
Produced by FastQC (version 0.11.5)