FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8949767
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95260.10643852515937006No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA134150.059.3335881
GGTATCA51700.054.161681
CAACGCA148600.051.478535
ATCAACG150950.050.846123
TCAACGC153100.050.0372774
ACGCAGA160650.047.6667
CGCAGAG161050.047.5526738
AACGCAG163500.046.9416736
GCAGAGT197150.038.881399
TCTGTCG28950.038.2315148
TATCAAC208650.037.298432
GTCTCGC25900.029.903941
CTCGCTC24350.029.3213183
CTGTCGC40650.027.5245349
CGCTCTG26500.027.385335
AGAGTAC219350.026.42861610-11
AGTACGG216600.025.9963612-13
GAGTACG198650.025.36402912-13
TCTCGCT30050.024.7495172
CAGAGTA221650.023.64366110-11
Produced by FastQC (version 0.11.5)