FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14431446
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC377970.26190722676022904No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC244840.16965728867363672No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC149990.10393275905962578No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA46950.053.3151634
GCGACAT47050.053.2018475
CTGCGAC54000.046.974033
CGACATC54950.045.4659466
GCTGCGA60050.042.2414252
GGCTGCG78900.032.9936521
CTAGGCG137200.028.1798367
GCGCGGT47850.027.9920926
TAGGCGG141300.027.3286428
TCGACCG10550.026.5650043
GCTAGGC155500.025.1689416
CCGCTCG11350.024.6901847
GGCTAGG169150.023.6717995
CGCGGTG58450.023.484517
TGGCTAG170300.023.4003854
TTGATCG56100.022.47913622-23
CGACCGC12900.021.7239114
GGGCGCG61950.021.6980554
CCGATAG62100.021.30167890-91
CCGGGCG64850.021.1688942
Produced by FastQC (version 0.11.5)