FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004913955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8752381
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG5372516.138341098268003No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA2960513.382519568103811No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG2692713.076545685111286No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG2216352.532282358366255No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1819572.078942861376807No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1379881.5765767052416935No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA1271721.4529989039553923No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA1063481.2150750749995916No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1047491.1968057606267368No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA1024991.1710984702334142No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1006991.1505326379187562No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA932251.0651387319633367No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG865670.9890680033238956No Hit
TGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA713190.814852552693947No Hit
GTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA641220.7326234998225055No Hit
GGTGGCAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA598640.6839738809359419No Hit
TGTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA573960.6557758397400661No Hit
CTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA526480.6015277442789567No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT510660.5834526627668517No Hit
TGGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA499560.5707703995061458No Hit
TGTGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG484700.5537921623841557No Hit
ATGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA462640.5285875923363026No Hit
TGGGGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG367900.42034276158681844No Hit
GTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG353110.4034445027016077No Hit
AGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG350510.40047388247837934No Hit
TGCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA337260.3853351448023115No Hit
AGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA318780.3642208902925958No Hit
TGGCTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA303070.34627148886685805No Hit
GTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA292310.3339776913276513No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN288870.3300473322630722No Hit
TGGTCGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG284180.32468879039886406No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG282640.32292926918972104No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA278820.3185647425540547No Hit
TGGTAATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG269600.30803046622399094No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC265590.30344885580278097No Hit
TTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG256660.29324591788223114No Hit
TGGTTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA244820.2797181704041449No Hit
GGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA233260.2665103358731756No Hit
CGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG216880.2477954284668366No Hit
TAGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG210760.24080304547985285No Hit
GCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG209570.23944341545460598No Hit
TGAGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA201600.23033732192417125No Hit
GGTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA201540.23026876914978905No Hit
TTGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA201330.23002883443945138No Hit
TAGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA194270.2219624579871466No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC190020.21710663646840786No Hit
TGGCTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG185480.2119194765401552No Hit
TTGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA182900.20897170724172084No Hit
TGGCCGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG181050.20685799669826987No Hit
TGGATGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA177360.20264200107376495No Hit
TGTTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG171120.1955125125380168No Hit
GGTTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG169800.19400435150160852No Hit
TGGCAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG164830.1883258966902835No Hit
TTGTGGAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC161290.18428128300173402No Hit
GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTAC160520.18340152239716256No Hit
GGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGTAA159000.18166485211281364No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC156290.1785685518032179Illumina Single End PCR Primer 1 (100% over 50bp)
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA155600.17778019489782265No Hit
TGATGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG155380.17752883472508796No Hit
TGCTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG149680.17101632115877954No Hit
TGGTTATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG148260.16939390549840094No Hit
TGTGGCAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA140160.1601392809568048No Hit
GGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG139570.15946517867537988No Hit
TTGTGTAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC136410.15585473255791765No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG135710.1550549501901254No Hit
TGTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA129140.1475484213952752No Hit
CTGGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGT127540.14572034741175No Hit
TGGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA125050.14287540727488898No Hit
CCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTC122910.14043035832192408No Hit
TCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG122510.13997333982604276No Hit
TGGCGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG119180.136168660847831No Hit
TGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTA116870.13352937903411655No Hit
GGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA115300.13173558143778247No Hit
CGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA114520.13084439537081394No Hit
GGCTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG113330.1294847653455671No Hit
GGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA112160.12814798624511434No Hit
GGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA112130.12811370985792322No Hit
TGGTCAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA111840.1277823714484093No Hit
GGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGG110840.12663982520870606No Hit
TGGTCAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA110420.12615995578803071No Hit
GATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCA108880.12440043457888773No Hit
TGGCGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG108100.12350924851191922No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA105540.12058433013827895No Hit
TGGGAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG104410.11929325288741428No Hit
TTGTGCAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC101350.1157970613939224No Hit
TTGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG99130.11326060874178123No Hit
GGGGTGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA98310.1123237208252246No Hit
TGGCTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA98060.11203808426529878No Hit
TGGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA95370.1089646348804971No Hit
TGGAGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG95130.10869042378296831No Hit
TGGGCAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA94190.10761643031764728No Hit
GGTGGAAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA92220.1053656142254319No Hit
TGGTTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC91630.10469151194400701No Hit
TGGTATTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG89710.1024978231637768No Hit
TGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAG89520.1022807393782332No Hit
TGGTCATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG88450.10105821490175074No Hit
TCGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA88010.10055549455628132No Hit
GAGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG87960.10049836724429614No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)