FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18093533
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC699780.38675697001796167No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC425860.23536586248799501No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC189430.10469486528695086No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG188230.10403164489765487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA66000.070.095274
GCGACAT68600.067.369365
CTGCGAC72200.064.080263
GCTGCGA79650.058.384592
CGACATC100400.046.1725436
GGCTGCG102150.046.0842131
CTAGGCG211550.034.6881187
TAGGCGG215500.034.05248
ACCCGAC37500.033.6940233
GCTAGGC239650.030.8587916
CCCGACT42300.029.8686054
CCGACTC42250.029.6791135
TGGCTAG254500.029.5811584
GGCTAGG254600.029.4202925
CGACTCC44650.027.76436
TTGATCG84200.027.6135422-23
TGATCGC84600.027.39883824-25
GACATCT171600.027.2359857
GGCTGGC287100.026.3406681
GATCGCC89300.025.930224-25
Produced by FastQC (version 0.11.5)