FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8925312
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT652140.7306635331067418No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307150.34413362804571984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295000.33052065854952745No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95740.10726795881197207No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105750.081.038461
GTATCAA306900.071.632331
ATCAACG409750.053.6518363
TCAACGC416700.052.8281064
CAACGCA417000.052.661665
AACGCAG437950.050.200896
TATCAAC456150.048.3772432
ACGCAGA471400.046.5616537
CGCAGAG474400.046.2797558
GCAGAGT518650.042.388639
TCTGTCG27150.035.5021368
AGAGTAC545200.031.9375810-11
GTCTCGC27250.031.6672651
CTCGCTC27300.029.6471483
CAGAGTA545900.028.09824210-11
CGCTCTG29050.027.8610175
TCTCGCT30950.027.6892342
AGTACTT450350.027.21613112-13
CTGTCGC36800.026.8392019
AGTACGG203200.026.10399612-13
Produced by FastQC (version 0.11.5)