FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8925312
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA96210.10779455104762724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132800.056.5105821
CAACGCA145950.050.0405775
GGTATCA55450.049.7320981
ATCAACG148800.048.922133
TCAACGC152600.047.663814
ACGCAGA159000.046.0929767
CGCAGAG160900.045.4028368
AACGCAG162550.045.2327166
GCAGAGT194050.037.283919
TATCAAC207000.035.7409062
TCTGTCG27750.034.5146568
GTCTCGC25100.031.0852891
TCTCGCT27850.027.355812
CTCGCTC24850.027.3061393
CTGTCGC37700.026.5136479
AGTACGG211550.025.9355212-13
AGAGTAC216400.025.86109710-11
GAGTACG194300.024.6394112-13
CGCTCTG27400.024.547645
CAGAGTA222850.022.57596810-11
Produced by FastQC (version 0.11.5)