FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16509284
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCCTCCTCCCTCACCGCTCCATGTGCGTCC571710.3462960598412384No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC258210.15640290638891427No Hit
GGCTAGGCGGGTGTCCCCCTCCTCCCTCACCGCTCCATGTGCGTCCCTCC252540.1529684751925038No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC197300.11950851411848024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA55500.053.1486784
CTGCGAC62450.047.3873673
GCGACAT63000.047.0475045
GCTGCGA70000.042.2764322
CTAGGCG249000.033.953587
TAGGCGG249750.033.8325968
TGGCTAG257350.033.7954334
CGACATC91150.032.464856
GCTAGGC262300.032.3237656
GGCTAGG273500.031.6955345
GGCTGCG99200.030.311651
GGCTGGC289800.029.8653431
CTGGCTA300400.029.0321873
GCTGGCT312150.027.7720412
AGGCGGG320800.026.5171079
CCGATAG113100.024.12755890-91
ACCCGAC26100.023.2957383
TCGACCG13050.023.2957383
CGGTCGA186950.022.68882268-69
CGACGGT33850.022.4526883
Produced by FastQC (version 0.11.5)