FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16509284
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG226040.13691690081774596No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG38550.048.1898165
CGGGGAT191400.041.970748
TCGGGGA199900.040.233387
CTGTCGG46950.038.3573534
GTCGGGG53600.036.3370446
CTATCGG110650.033.6244664
TCTATCG107550.033.4514353
TCTGTCG54000.033.444073
ATCGGGG147500.032.914666
TATCGGG121850.032.2075275
GTCCCGT24950.031.6199761
CCCGTTA17250.028.3777123
GGGGATG287300.028.060239
CCCGTTT53800.026.5013643
CCCCGTT49050.026.2575552
GTTCCGT24450.026.0465341
CCCCGAA39400.025.9338052
CCGTTTA24300.025.8114684
GCCCCGT38600.025.6094361
TCCGACC28800.025.567269
Produced by FastQC (version 0.11.5)