FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20034611
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC60350.052.341129
TTCCGAC63950.049.618338
GCTCCGT67300.037.761411
CGGGGAT101050.037.183178
GTTTCCG78100.036.918586
CCGTTTC93900.036.6252254
CTCCGTT78500.036.6087952
TTTCCGA87950.036.402517
TCCGTTT100650.035.206743
CGTTTCC88150.034.1105465
CGATCGG93200.033.84366286-87
GTCCCGT30050.033.3539661
TCGGGGA114500.033.2717937
ATAGCGC94900.033.16066494-95
CCGATCG97150.032.80990286-87
CTATCGG55900.031.4410634
CCCCGTT61300.031.3054962
ACCCGAT103050.030.90828584-85
GCCCCGT44350.030.7395671
CGGCATA103150.030.6455790-91
Produced by FastQC (version 0.11.5)