FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9189817
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC1052981.1458117174694555No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC584660.6362041812149252No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG146000.15887149874692824No Hit
TCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCA129080.14045981546748973No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA122820.1336479279184776No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT73000.079.177215
TGCGACA73250.078.647634
CTGCGAC76100.075.889453
GCTGCGA77200.074.6235662
CGACATC80100.072.090696
GGCTGCG87600.065.982441
CTAGGCG211850.053.4162867
TAGGCGG213600.053.0228238
GCTAGGC225350.050.237366
TCAGACG27650.049.8121535
GACATCT119450.048.819167
GGCTAGG234350.048.6383365
TGGCTAG241650.047.30664
CAGACGT30500.045.6190156
GGCTGGC251200.045.603311
AGGCGGG250750.045.2045869
ACATCTG129850.044.9454428
GCTGGCT260450.044.2748152
CTGGCTA272350.042.5669823
CATCTGT144150.040.32169
Produced by FastQC (version 0.11.5)