FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9189817
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG259840.2827477413315194No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG147680.16069960914346826No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG138890.15113467439014291No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC49350.064.102569
TTCCGAC50700.062.491088
TGTCGGG26800.056.560345
TTTCCGA57450.055.56317
CTGTCGG28250.052.3164564
GTTTCCG52150.051.1907656
CGTTTCC56800.046.0119365
TCGGGGA159700.045.240937
CCGTTTC55700.044.9626354
GCTCCGT39950.044.961241
GTCGGGG35800.044.3172766
CTCCGTT44050.044.230082
CGGGGAT163550.044.117148
TCCGTTT55250.042.231233
TCTGTCG35850.041.3570863
GTTCCGT13450.039.5693551
GTCCCGT11550.037.850281
GGTGTGC77200.036.8569266
GTGCTCT100950.036.277429
ATCGGGG116700.036.063886
Produced by FastQC (version 0.11.5)