FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12293323
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC394220.3206781437370514Illumina Single End PCR Primer 1 (100% over 50bp)
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG265950.21633694974092849No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC66600.069.608729
TTCCGAC70100.066.134598
TTTCCGA80700.058.036617
GTTTCCG69250.057.892376
CTCCGTT61850.052.3179782
CGTTTCC74950.052.3014225
GCTCCGT58350.052.279191
CCGTTTC77750.050.3606764
TCCGTTT78850.048.5711823
GTTCCGT17450.039.7550351
CCCCGTT27950.036.891092
CCCCCGT23550.034.299891
CGACCTG70800.033.97988510-11
CCCGTTA15150.033.8732153
GCCCCGT21550.033.0733871
CCGACCT73200.032.7360110-11
GCCGGTT74650.032.4480518-19
GTCCCGT15850.032.3764461
CCCCGTA16100.031.284092
GTCCGTT14450.030.9103242
Produced by FastQC (version 0.11.5)