FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15313360
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC1560821.0192537757879394No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC979110.6393828656806867No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG241220.1575225815888871No Hit
TCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCA217110.14177815972458038No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA200190.1307289843639802No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA121400.073.195024
GCGACAT121500.073.170155
TCGGTCG199950.072.07770568-69
CTGCGAC125350.070.922813
GCTGCGA128400.069.171382
CGACATC128600.069.081446
GGCTGCG146800.060.960371
GGACGAC121900.059.67507678-79
AGGACGA136300.053.4023178-79
GAGGACG139250.052.46316576-77
GACATCT183600.048.668027
TCAGACG48450.047.91375
CTAGGCG320300.046.548887
TAGGCGG322150.046.2815678
CAGACGT53200.044.4337466
GCTAGGC340700.043.8373766
ACATCTG206350.043.4065368
GGCTAGG355100.042.33555
TGGCTAG361600.041.7171554
GGCTGGC384950.039.1883351
Produced by FastQC (version 0.11.5)