FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16690967
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATG726110.43503171505880994TruSeq Adapter, Index 10 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG12500.041.2677153
GCTCGCG14300.036.366169
CCGCTCG16950.031.1877147
GCGCGGT43750.027.997226
CGACCGC20150.026.6669984
GGGCGCG47400.026.1188054
CGCGGTG47550.025.7597987
CGCGTCG10350.025.33014112-13
TCGGTCG14450.024.98520968-69
ATGCGGT29500.024.3351295
TCGCGTC10600.024.32727810-11
CTCGACC22150.023.2887782
CTCGCGT11350.023.0984110-11
AATGCGG31000.023.0189554
CGTCGCA11700.022.77476514-15
GACCGCT23300.022.6928165
GGCGCGG55450.022.6370855
CGCTCGC23150.022.278118
CCTCGAC25000.021.4998511
TCCGATC66600.021.4248216-17
Produced by FastQC (version 0.11.5)