FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8461670
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT608380.718983368531271No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287920.34026380135363354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276650.32694491749264626No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96070.11353550776619745No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98450.080.1810151
GTATCAA286050.071.932471
ATCAACG381250.053.954663
CAACGCA385400.053.4190335
TCAACGC386350.053.2424284
AACGCAG404600.050.841746
TATCAAC421950.048.990022
ACGCAGA436700.047.072617
CGCAGAG441400.046.625278
GCAGAGT481600.042.782779
TCTGTCG27450.039.8591088
AGAGTAC502650.032.08910-11
CTGTCGC35250.031.3766029
CAGAGTA507400.028.2317910-11
GTCTCGC23950.027.5698131
ACGGGAC17600.027.3770163
AGTACGG191100.026.6824412-13
CTCGCTC24650.026.3040963
AGTACTT419700.026.3031712-13
GAGTACG173550.024.9949812-13
Produced by FastQC (version 0.11.5)