FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9311112
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC301620.32393552993455565Illumina Single End PCR Primer 1 (100% over 50bp)
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG152910.164223134680369No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC41450.063.2568639
TTCCGAC43350.060.5942548
GTTTCCG41950.054.1250536
TTTCCGA49900.053.1173067
CTCCGTT38250.050.4388282
GCTCCGT36000.050.159031
CCGTTTC45900.049.176324
CGTTTCC46750.047.767555
TCCGTTT49250.047.4716073
CGGTGTG119300.040.6632465
GTGCTCT544650.038.861619
GGTGTGC345600.038.731986
TGTGCTC549600.038.485888
GTTCCGT11000.038.4472581
GTGTGCT553700.038.3044627
CCGGTGT57300.037.1533934
GTCCCGT12750.034.660981
ACGGTGT37250.034.5714874
ACCGGTG20100.033.334823
CCCCCGT17100.033.068841
Produced by FastQC (version 0.11.5)