FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15339588
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC373490.24348111565969047Illumina Single End PCR Primer 1 (100% over 50bp)
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG244550.15942409926524753No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC68450.060.033649
TTCCGAC72900.056.434138
GTTTCCG68200.053.434816
TTTCCGA81800.050.3519947
CCGTTTC76050.048.873444
GCTCCGT57550.048.595081
CGTTTCC75150.048.006415
CTCCGTT64800.047.7545742
TCCGTTT82300.046.4277953
CGGTGTG188450.043.905195
CCGGTGT93100.042.1663674
GGTGTGC536250.041.137676
GTGCTCT843350.040.5730749
GTGTGCT848150.040.3770267
TGTGCTC844350.040.3508078
CCCCCGT31550.037.172341
GTTCCGT20100.035.9062351
GCGGTGT49300.035.6258434
CCCCGTT37450.034.4714432
ACGGTGT58850.033.4743354
Produced by FastQC (version 0.11.5)