FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004913988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16471977
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG2710051.6452487761487282TruSeq Adapter, Index 22 (97% over 37bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTAT333670.2025682770198137TruSeq Adapter, Index 22 (97% over 37bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTAT256040.15543975079615518TruSeq Adapter, Index 22 (97% over 37bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTA254160.15429841845942355TruSeq Adapter, Index 22 (97% over 37bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTAT211130.12817526396497517TruSeq Adapter, Index 22 (97% over 37bp)
ACAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCT210600.1278535053806838TruSeq Adapter, Index 22 (97% over 37bp)
ACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTC204320.12404096970266533TruSeq Adapter, Index 22 (97% over 37bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCT201870.12255359511490332TruSeq Adapter, Index 22 (97% over 37bp)
AGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTC191910.1165069620968995TruSeq Adapter, Index 22 (97% over 37bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTA179600.10903366365798108TruSeq Adapter, Index 22 (97% over 37bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTA178340.10826872815570347TruSeq Adapter, Index 22 (97% over 37bp)
ACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCG174630.10601641806566389TruSeq Adapter, Index 22 (97% over 37bp)
CAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCT174180.10574322681485046TruSeq Adapter, Index 22 (97% over 37bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTC171660.10421335581029527TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)