FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16471977
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2630001.5966510880873621Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC321300.19505855308078685Illumina Single End PCR Primer 1 (100% over 49bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC249680.15157864778465874Illumina Single End PCR Primer 1 (100% over 49bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG246170.14944775602831403Illumina Single End PCR Primer 1 (100% over 48bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC206130.12513980562260377Illumina Single End PCR Primer 1 (100% over 49bp)
GGCTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG202590.12299070111620482Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG197780.12007059019084351Illumina Single End PCR Primer 1 (100% over 45bp)
GGCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG194230.11791541476775982Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG186610.11328937625398579Illumina Single End PCR Primer 1 (100% over 45bp)
GCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG174160.10573108498148098Illumina Single End PCR Primer 1 (100% over 48bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG172900.10496614947920338Illumina Single End PCR Primer 1 (100% over 48bp)
GGCCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG169150.10268955572242482Illumina Single End PCR Primer 1 (100% over 44bp)
GTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT167720.10182141463650661Illumina Single End PCR Primer 1 (100% over 46bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTTA27000.064.055661
GGCCGTA33800.051.7310071
GCGTTAG47500.046.9144862
CCCGAGA50200.045.338123
CGTTAGA76000.044.639193
GCCGTTA24800.044.0652241
GGCGTAG54700.043.6049961
GCGGCTA15200.042.8249471
GCCGCGA10000.039.91161
CCGTAGA66300.039.4167563
CCGTTAG26750.039.255112
CGGTGTG109150.039.220135
CCGAGAT105100.038.655354
CGCGAGA19050.038.161743
GGTGTGC302000.037.8909036
CGTGAGA79950.037.382123
CCGGTGT55100.037.3407064
GGCCCGA23250.037.1936041
GTGCTCT472450.036.9484379
GTGTGCT474650.036.8281147
Produced by FastQC (version 0.11.5)