FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12954892
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATG1514361.1689483787282828TruSeq Adapter, Index 1 (97% over 37bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTAT197400.15237487120695412TruSeq Adapter, Index 1 (97% over 37bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTA163460.12617627379680202TruSeq Adapter, Index 1 (97% over 37bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCT139410.1076118581305039TruSeq Adapter, Index 1 (97% over 37bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTAT137910.10645399436753313TruSeq Adapter, Index 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG8950.042.4526863
GCTCGCG10350.038.082529
CGCCAGA103150.034.9470673
ACGCCAG77500.032.4184462
GCCAGAT425500.031.4765974
CCGCTCG12800.031.1644127
AACGCCA36650.029.9566441
CGCGTCG7300.026.34673312-13
CGACCGC16150.025.8790534
CCAGATC944050.025.4863955
CACGCCA49050.025.096661
CGTCGCA8100.024.62420814-15
CGCTCGC16350.024.3977328
ACGGCCA32800.023.7643091
TCGCGTC8100.023.7442210-11
TGCTTGA2565850.023.42566364-65
CTTGAAA2548200.023.3517666-67
AACGCAG40150.023.3203931
TACGCCA19650.023.2200891
TCTGCTT2631050.023.0618362-63
Produced by FastQC (version 0.11.5)