FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12954892
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1474181.1379330680641722Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC190660.14717220336533876Illumina Single End PCR Primer 1 (100% over 49bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG159120.1228261879759399Illumina Single End PCR Primer 1 (100% over 48bp)
GGCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG134460.10379090771270034Illumina Single End PCR Primer 1 (100% over 44bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC133300.10289549306933628Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTTA20450.052.0493471
GGTGTGC224500.043.3750956
CGGTGTG80200.042.3018345
GGCCGTA21150.041.789111
CCGGTGT45300.040.910874
CGTTAGA46150.040.8776633
GTGCTCT373550.039.5204479
TGTGCTC371950.039.512428
GCCGTTA15350.039.005221
GCGTTAG32250.038.604582
GGCGTAG39100.034.6360741
CCGTTAG17200.034.2578852
GTGTGCT442550.034.196877
CGTGAGA56700.034.1097563
CCGTAGA40100.033.6543543
CCCCCGT23600.032.6184651
GTCCCGT17700.032.4842341
CCCGAGA34100.032.190293
CCGTTTA17150.032.1415024
GCCGTAG34050.031.6793332