FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8924210
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT615040.689181451355358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295170.330751965720215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267980.3002842828664946No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG111390.12481777098477065No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98600.076.8411561
GTATCAA262650.066.4172441
ATCAACG330050.052.978233
TCAACGC331750.052.670874
CAACGCA332250.052.6453445
AACGCAG349500.050.172236
ACGCAGA384500.045.5407377
CGCAGAG388150.045.1124888
TATCAAC389650.044.99852
GCAGAGT429700.040.7780159
AGAGTAC455450.029.19731910-11
GCAGCGC16900.028.5170388
CAGAGTA451150.027.9589610-11
AGTACTT445350.024.94969712-13
ACGGGAC14300.024.555083
GTCGTGC8800.023.671611
GAGTACG86000.023.59185212-13
GAGTACT449100.023.56888412-13
AGTACGG96300.023.41634212-13
TGCAGCG19800.022.5373177
Produced by FastQC (version 0.11.5)