FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8924210
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG115930.12990505602176552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA70550.046.2097475
ACGCAGA73950.044.3041427
TCAACGC74350.043.703644
CGCAGAG75500.043.025418
ATCAACG77950.041.3821953
AACGCAG82150.039.95336
GTATCAA89400.038.2362441
GGTATCA38450.032.0608371
GCAGAGT102150.032.0357789
GCAGCGC15300.026.0532958
AGTACGG99200.023.9648412-13
GAGTACG89050.023.82292212-13
TATCAAC147450.022.724482
ACGGGAC16900.022.5384273
TACGGGT16250.021.2412764
GTACGGG109250.020.4414021
TGCAGCG20300.019.6257
GCTCTAG23250.019.4666631
AGAGTAC125950.019.29788410-11
AATGCGT28350.019.2954966
Produced by FastQC (version 0.11.5)