FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004914000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11345755
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATG972080.856778592522049TruSeq Adapter, Index 7 (97% over 37bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTAT161550.14238805614963482TruSeq Adapter, Index 7 (97% over 37bp)
AACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAA122340.10782887520486738No Hit
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTA117200.1032985464607688TruSeq Adapter, Index 7 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph