FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11345755
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC950420.837687751938941Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC155880.1373905923404833Illumina Single End PCR Primer 1 (100% over 49bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG113580.10010792582776554Illumina Single End PCR Primer 1 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC34100.045.411239
TTCCGAC34700.044.6270038
GGCCGTA15550.041.250551
GTTCCGT15600.038.6816941
GGCGTTA11600.036.455111
TTTCCGA43650.036.129947
GTTTCCG37800.033.804456
CTCCGTT35050.033.484882
CCCGTTA11400.033.34473
CCCCGTT22400.033.0914962
GTCCCGT13150.032.5194471
CGGTGTG35100.031.9472275
GCTCCGT31000.031.4208951
CCGTTAT9950.030.5642624
CCGGTGT22050.030.385584
TCCGTTT46450.030.0748393
CCGTTTC44350.030.000014
GTGCTCT131950.029.7711859
CCGTTAA11700.029.6479034
CGTTTCC42750.029.2312855
Produced by FastQC (version 0.11.5)