FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8944526
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT634060.7088804929405985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305120.34112483993003095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270950.3029227037855332No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG113120.12646841207683895No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99000.076.77821
GTATCAA269950.067.03051
ATCAACG338200.053.9410823
TCAACGC339350.053.723214
CAACGCA339700.053.6675535
AACGCAG358000.050.9761776
ACGCAGA391300.046.6676867
TATCAAC393450.046.3531232
CGCAGAG396450.046.074158
GCAGAGT438050.041.7529879
AGAGTAC460800.029.78162210-11
GCAGCGC18200.029.4224288
CAGAGTA457700.028.39739810-11
TGCAGCG20800.025.4598547
AGTACTT455500.025.16448212-13
GAGTACG88250.024.03544612-13
AGTACGG98350.023.8660212-13
GAGTACT456900.023.82421712-13
ACGGGAC13550.023.2808783
GTCGTGC9300.021.7609651
Produced by FastQC (version 0.11.5)