FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16792356
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC461090.27458326872060124No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC263390.15685112916853358No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC217330.12942198224001444No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT46850.057.4831165
TGCGACA48900.054.9768144
CTGCGAC55100.049.3103873
GCTGCGA60400.044.9049762
CGACATC63650.042.2362026
GGCTGCG80750.034.2952731
CTAGGCG161150.031.5659987
TAGGCGG167750.030.3523758
ACCCGAC32250.027.3953743
GCTAGGC188850.027.2381946
TGGCTAG198200.026.840244
GGCTAGG196750.026.4824875
CCGACTC35250.024.3885675
GCGCGGT61500.023.9416146
TTGATCG57150.023.68690922-23
CGACTCC37450.023.5900026
CCCGACT37800.023.3718554
TCGATCG19550.022.59431516-17
GGCTGGC234800.022.4762671
AGGCGGG230250.022.2990559
Produced by FastQC (version 0.11.5)