FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5230827
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATG59493111.373555271470458TruSeq Adapter, Index 23 (97% over 38bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTAT1097942.0989797597970647TruSeq Adapter, Index 23 (97% over 38bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTA480660.9188986751043382TruSeq Adapter, Index 23 (97% over 38bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTAT429080.8202909406103471TruSeq Adapter, Index 23 (97% over 38bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTAT378720.7240155332990367TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC342870.6554795255128874TruSeq Adapter, Index 23 (97% over 38bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTA333930.6383885377971781TruSeq Adapter, Index 23 (97% over 38bp)
GAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCCGTCT289650.5537365315274239RNA PCR Primer, Index 29 (97% over 36bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTA287440.5495115781883055TruSeq Adapter, Index 23 (97% over 38bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTA285260.5453439771569581TruSeq Adapter, Index 23 (97% over 38bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC255840.4891004806696914TruSeq Adapter, Index 23 (97% over 38bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTAT229720.4391657380372167TruSeq Adapter, Index 23 (97% over 38bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT198900.3802458005206442TruSeq Adapter, Index 23 (97% over 38bp)
ACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTA189670.36260040716315034TruSeq Adapter, Index 23 (97% over 38bp)
CCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC184780.353251980996504TruSeq Adapter, Index 23 (97% over 38bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTA180620.3452991276522814TruSeq Adapter, Index 23 (97% over 38bp)
ACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG168000.3211729235166829TruSeq Adapter, Index 23 (97% over 38bp)
CACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT167890.32096263172152323TruSeq Adapter, Index 23 (97% over 38bp)
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT165590.3165656214590924TruSeq Adapter, Index 23 (97% over 38bp)
ACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC162500.3106583337586963TruSeq Adapter, Index 23 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATATCGTATG157990.30203637015714724TruSeq Adapter, Index 23 (97% over 38bp)
ACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG155420.297123189124779TruSeq Adapter, Index 23 (97% over 38bp)
CCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG154430.2952305629683413TruSeq Adapter, Index 23 (97% over 38bp)
CCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT153300.29307029270897317TruSeq Adapter, Index 23 (97% over 38bp)
GCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT153170.2928217660419662TruSeq Adapter, Index 23 (97% over 38bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG148670.2842189198763408TruSeq Adapter, Index 23 (97% over 38bp)
CCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC145290.2777572265341599TruSeq Adapter, Index 23 (97% over 38bp)
AGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC145280.27773810909823626TruSeq Adapter, Index 23 (97% over 38bp)
ACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT145090.27737487781568765TruSeq Adapter, Index 23 (97% over 38bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT142890.27316904191249297TruSeq Adapter, Index 23 (97% over 38bp)
GCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG139220.26615294292852737TruSeq Adapter, Index 23 (97% over 38bp)
CCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC137560.2629794485652078TruSeq Adapter, Index 23 (97% over 38bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT127540.24382377776974848TruSeq Adapter, Index 23 (97% over 38bp)
CAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG127390.24353701623089427TruSeq Adapter, Index 23 (97% over 38bp)
CAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC125500.23992382084133157TruSeq Adapter, Index 23 (97% over 38bp)
CAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT125390.23971352904617182TruSeq Adapter, Index 23 (97% over 38bp)
CCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC125220.23938853263547047TruSeq Adapter, Index 23 (97% over 38bp)
CGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG123410.23592827673329667TruSeq Adapter, Index 23 (97% over 38bp)
CCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC119890.2291989392881852TruSeq Adapter, Index 23 (97% over 38bp)
AGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC113710.21738436388739293TruSeq Adapter, Index 23 (97% over 38bp)
AGCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT110840.21189765977731628TruSeq Adapter, Index 23 (97% over 38bp)
CCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT110130.21054032182673982TruSeq Adapter, Index 23 (97% over 38bp)
GTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTA109280.2089153397732328TruSeq Adapter, Index 23 (97% over 38bp)
GCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT103900.1986301592463295TruSeq Adapter, Index 23 (97% over 38bp)
CGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC102190.19536107770339184TruSeq Adapter, Index 23 (97% over 38bp)
CCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT101410.1938699177013501TruSeq Adapter, Index 23 (97% over 38bp)
CACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC100500.1921302310323014TruSeq Adapter, Index 23 (97% over 38bp)
CGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC98170.18767586846209977TruSeq Adapter, Index 23 (97% over 38bp)
AGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT94630.1809082961451411TruSeq Adapter, Index 23 (97% over 38bp)
TGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC93970.1796465453741827TruSeq Adapter, Index 23 (97% over 38bp)
CAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT92230.17632011152347418TruSeq Adapter, Index 23 (97% over 38bp)
CACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT92140.1761480546001617TruSeq Adapter, Index 23 (97% over 38bp)
CCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC90240.1725157417746754TruSeq Adapter, Index 23 (97% over 38bp)
ACCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT89160.1704510586949253TruSeq Adapter, Index 23 (97% over 38bp)
CCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC88600.16938048228320302TruSeq Adapter, Index 23 (97% over 38bp)
GCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG87500.1672775643316057TruSeq Adapter, Index 23 (97% over 38bp)
TGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC87020.16635992740727232TruSeq Adapter, Index 23 (97% over 38bp)
GCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT86570.16549964279070978TruSeq Adapter, Index 23 (97% over 38bp)
CTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT86400.16517464638000837TruSeq Adapter, Index 23 (97% over 38bp)
CCACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT85210.16289967150509851TruSeq Adapter, Index 23 (97% over 38bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT84440.1614276289389804TruSeq Adapter, Index 23 (97% over 38bp)
CCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG83120.15890412739706358TruSeq Adapter, Index 23 (97% over 38bp)
GACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT80560.1540100638006189TruSeq Adapter, Index 23 (97% over 38bp)
CCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT80550.1539909463646953TruSeq Adapter, Index 23 (97% over 38bp)
ACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT80520.15393359405692447TruSeq Adapter, Index 23 (97% over 38bp)
ACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT78630.1503203986673618TruSeq Adapter, Index 23 (97% over 38bp)
AACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC77120.14743366584289636TruSeq Adapter, Index 23 (97% over 38bp)
AGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG77090.1473763135351255TruSeq Adapter, Index 23 (97% over 38bp)
ACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG76810.14684102532926438TruSeq Adapter, Index 23 (97% over 38bp)
CCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC75850.1450057514805976TruSeq Adapter, Index 23 (97% over 38bp)
ACCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC74740.14288371609307668TruSeq Adapter, Index 23 (97% over 38bp)
GCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC72700.13898375916465983TruSeq Adapter, Index 23 (97% over 38bp)
CACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG72560.13871611506172926TruSeq Adapter, Index 23 (97% over 38bp)
CACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT70830.13540879864694436TruSeq Adapter, Index 23 (97% over 38bp)
CTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT70050.1339176386449026TruSeq Adapter, Index 23 (97% over 38bp)
CACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC69390.13265588787394422TruSeq Adapter, Index 23 (97% over 38bp)
ACCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT68850.13162354633406917TruSeq Adapter, Index 23 (97% over 38bp)
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT67740.12950151094654824TruSeq Adapter, Index 23 (97% over 38bp)
AGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT67110.12829711248336068TruSeq Adapter, Index 23 (97% over 38bp)
CAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC66740.127589767354187TruSeq Adapter, Index 23 (97% over 38bp)
ACCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC66100.12636625145507585TruSeq Adapter, Index 23 (97% over 38bp)
ACACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC65830.1258500806851383TruSeq Adapter, Index 23 (97% over 38bp)
GGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG65620.12544861453074246TruSeq Adapter, Index 23 (97% over 38bp)
CCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC65560.12533390991520077TruSeq Adapter, Index 23 (97% over 38bp)
ATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTA65110.12447362529863824TruSeq Adapter, Index 23 (97% over 38bp)
CATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT64700.12368981042577015TruSeq Adapter, Index 23 (97% over 38bp)
TGCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT64600.12349863606653402TruSeq Adapter, Index 23 (97% over 38bp)
TGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTA64020.12238982478296452TruSeq Adapter, Index 23 (97% over 38bp)
TGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG62770.12000014529251302TruSeq Adapter, Index 23 (97% over 38bp)
TGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG62370.11923544785556854TruSeq Adapter, Index 23 (97% over 38bp)
GGCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC62070.11866192477786018TruSeq Adapter, Index 23 (97% over 38bp)
CCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG61420.1174192914428254TruSeq Adapter, Index 23 (97% over 38bp)
CTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG60470.11560313503008225TruSeq Adapter, Index 23 (97% over 38bp)
ACCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC60280.11523990374753361TruSeq Adapter, Index 23 (97% over 38bp)
GGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG58730.11227670117937374TruSeq Adapter, Index 23 (97% over 38bp)
CCCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT58360.1115693560502001TruSeq Adapter, Index 23 (97% over 38bp)
CCCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT58320.11149288630650565TruSeq Adapter, Index 23 (97% over 38bp)
AACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC58060.11099583297249174TruSeq Adapter, Index 23 (97% over 38bp)
CGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT57690.11028848784331807TruSeq Adapter, Index 23 (97% over 38bp)
CCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG57460.10984878681707501TruSeq Adapter, Index 23 (97% over 38bp)
CCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT57150.10925614630344303TruSeq Adapter, Index 23 (97% over 38bp)
CCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT56960.1088929150208944TruSeq Adapter, Index 23 (97% over 38bp)
CGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT56850.10868262322573467TruSeq Adapter, Index 23 (97% over 38bp)
ACGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT56710.1084149791228041TruSeq Adapter, Index 23 (97% over 38bp)
ACTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC56670.10833850937910965TruSeq Adapter, Index 23 (97% over 38bp)
ACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT56150.10734440271108181TruSeq Adapter, Index 23 (97% over 38bp)
CAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG55590.10627382629935955TruSeq Adapter, Index 23 (97% over 38bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG55580.10625470886343592TruSeq Adapter, Index 23 (97% over 38bp)
TGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT55380.10587236014496369TruSeq Adapter, Index 23 (97% over 38bp)
ACCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT55100.10533707193910255TruSeq Adapter, Index 23 (97% over 38bp)
AACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT54760.10468707911769976TruSeq Adapter, Index 23 (97% over 38bp)
AGCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT54630.10443855245069278TruSeq Adapter, Index 23 (97% over 38bp)
AAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC54340.10388414680890803TruSeq Adapter, Index 23 (97% over 38bp)
CGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG53480.1022400473194774TruSeq Adapter, Index 23 (97% over 38bp)
ACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG53420.10212534270393572TruSeq Adapter, Index 23 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGG29900.072.753027
CCCCCCA22100.071.804581
CCGCCCC4350.068.809631
CCCGCCC5000.068.416431
ACCCCCA26500.068.129581
TAGCCCC4550.067.873441
CCCGCCA27650.067.186121
GGGTGTG18150.066.729886
CCCGGCC5000.066.5159761
GTGTGGA37450.066.076478
CCTGCCA21350.065.648091
CGGTGTG10700.064.36376
GGCCCAA8200.064.314231
CCCCGCC4000.064.14041
GCCCCAA15850.063.2486341
GCCCGCA5500.063.0515562
ACGGTGT5150.062.7131235
TGTGGAA39550.061.847439
CCCGCTA7600.060.6395651
CCGGCCC3850.060.469071
Produced by FastQC (version 0.11.5)