FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004914023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5230827
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4732599.047498607772729Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC845081.6155762750326097Illumina Single End PCR Primer 1 (100% over 49bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG367290.7021643040383481Illumina Single End PCR Primer 1 (100% over 48bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC341490.6528413193554289Illumina Single End PCR Primer 1 (100% over 49bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC296700.5672143238535704Illumina Single End PCR Primer 1 (100% over 49bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG261430.49978712735099057Illumina Single End PCR Primer 1 (100% over 48bp)
GCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG227190.43432902674854285Illumina Single End PCR Primer 1 (100% over 48bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG219370.4193791918562782Illumina Single End PCR Primer 1 (100% over 48bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCC213330.4078322605584165Illumina Single End PCR Primer 1 (98% over 50bp)
GGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG199010.38045609231580396Illumina Single End PCR Primer 1 (100% over 45bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGCC183840.3514549420196844Illumina Single End PCR Primer 1 (98% over 50bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC181970.347879981501969Illumina Single End PCR Primer 1 (100% over 49bp)
GCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC153570.2935864634789107Illumina Single End PCR Primer 1 (100% over 47bp)
GTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG152380.29131148860400086Illumina Single End PCR Primer 1 (100% over 48bp)
GGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG146500.280070436280917Illumina Single End PCR Primer 1 (100% over 45bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG142660.2727293408862499Illumina Single End PCR Primer 1 (100% over 48bp)
GTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC132940.254147193168499Illumina Single End PCR Primer 1 (100% over 47bp)
GGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT128640.24592669572134576Illumina Single End PCR Primer 1 (100% over 46bp)
GGCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG124010.23707532288871339Illumina Single End PCR Primer 1 (100% over 45bp)
GGCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG121740.23273566493405345Illumina Single End PCR Primer 1 (100% over 44bp)
GGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC120320.23002098903290055Illumina Single End PCR Primer 1 (100% over 47bp)
GGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG120090.22958128800665747Illumina Single End PCR Primer 1 (100% over 45bp)
TGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC119830.22908423467264352Illumina Single End PCR Primer 1 (100% over 47bp)
GCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT119230.2279371885172268Illumina Single End PCR Primer 1 (100% over 46bp)
GTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT116440.22260342389453905Illumina Single End PCR Primer 1 (100% over 46bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT114990.21983139568561527Illumina Single End PCR Primer 1 (100% over 46bp)
GTGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG114140.21820641363210824Illumina Single End PCR Primer 1 (100% over 45bp)
GGGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG110330.21092267054521208Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT110120.21052120439081623Illumina Single End PCR Primer 1 (100% over 46bp)
GCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG109980.21025356028788567Illumina Single End PCR Primer 1 (100% over 45bp)
GGCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG109470.20927857105578143Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG100480.19209199616045416Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG100350.19184346949344722Illumina Single End PCR Primer 1 (100% over 44bp)
GCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT99740.19067730590210688Illumina Single End PCR Primer 1 (100% over 46bp)
GGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG96080.18368032435406484Illumina Single End PCR Primer 1 (100% over 45bp)
GGCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG95270.18213181204425227Illumina Single End PCR Primer 1 (100% over 45bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCC94260.18020095101596745Illumina Single End PCR Primer 1 (98% over 50bp)
GGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG93390.1785377340906132Illumina Single End PCR Primer 1 (100% over 45bp)
GGCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG93000.17779215408959234Illumina Single End PCR Primer 1 (100% over 45bp)
GGCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT92780.17737157049927285Illumina Single End PCR Primer 1 (100% over 46bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCC90990.1739495494689463Illumina Single End PCR Primer 1 (98% over 50bp)
ACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG88010.1682525535637099Illumina Single End PCR Primer 1 (100% over 48bp)
GGGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG86310.16500258945669585Illumina Single End PCR Primer 1 (100% over 44bp)
GGCAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG82860.15840707406304969Illumina Single End PCR Primer 1 (100% over 44bp)
AGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC80130.1531880140559036Illumina Single End PCR Primer 1 (100% over 47bp)
GGGCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG79880.1527100781578133Illumina Single End PCR Primer 1 (100% over 44bp)
GGGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG79360.15171597148978547Illumina Single End PCR Primer 1 (100% over 45bp)
GGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG79240.15148656225870213Illumina Single End PCR Primer 1 (100% over 45bp)
GGGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG77730.1485998294342367Illumina Single End PCR Primer 1 (100% over 45bp)
GCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC77720.14858071199831308Illumina Single End PCR Primer 1 (100% over 47bp)
GGGCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG77620.14838953763907695Illumina Single End PCR Primer 1 (100% over 45bp)
GGGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG73680.1408572678851738Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG72840.13925140326759036Illumina Single End PCR Primer 1 (100% over 45bp)
TGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG70590.13494998018477766Illumina Single End PCR Primer 1 (100% over 45bp)
GGCGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG70430.1346441012099999Illumina Single End PCR Primer 1 (100% over 44bp)
GCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG69800.1334397027468123Illumina Single End PCR Primer 1 (100% over 45bp)
GGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG68910.13173825094961084Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG68610.13116472787190248Illumina Single End PCR Primer 1 (100% over 44bp)
TGGGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG68060.1301132688961038Illumina Single End PCR Primer 1 (100% over 44bp)
AGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT65770.12573537606959664Illumina Single End PCR Primer 1 (100% over 46bp)
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC65760.12571625863367303Illumina Single End PCR Primer 1 (100% over 47bp)
GGGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG65710.12562067145405498Illumina Single End PCR Primer 1 (100% over 44bp)
GTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC63670.1217207145256381Illumina Single End PCR Primer 1 (100% over 47bp)
GGCCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG63210.12084131247315195Illumina Single End PCR Primer 1 (100% over 44bp)
GGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC62520.11952220939442271Illumina Single End PCR Primer 1 (100% over 47bp)
AGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC62470.11942662221480466Illumina Single End PCR Primer 1 (100% over 47bp)
GCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT62300.11910162580410326Illumina Single End PCR Primer 1 (100% over 46bp)
GGGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG61580.11772517041760318Illumina Single End PCR Primer 1 (100% over 45bp)
GGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT60870.11636783246702673Illumina Single End PCR Primer 1 (100% over 46bp)
GCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG58910.11262081502599874Illumina Single End PCR Primer 1 (100% over 45bp)
GCAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG58420.11168406066574177Illumina Single End PCR Primer 1 (100% over 45bp)
GTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT58260.11137818169096396Illumina Single End PCR Primer 1 (100% over 46bp)
TGGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG57960.1108046586132556Illumina Single End PCR Primer 1 (100% over 45bp)
GGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT55350.10581500783719286Illumina Single End PCR Primer 1 (100% over 46bp)
GCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC55130.1053944242468734Illumina Single End PCR Primer 1 (100% over 47bp)
GGGCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG55120.10537530681094978Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG54990.10512678014394283Illumina Single End PCR Primer 1 (100% over 44bp)
GCGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG54390.10397973398852611Illumina Single End PCR Primer 1 (100% over 45bp)
GAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC53770.10279445296126216Illumina Single End PCR Primer 1 (100% over 47bp)
GCCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG52810.10095917911259539Illumina Single End PCR Primer 1 (100% over 45bp)
GCGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG52690.10072976988151204Illumina Single End PCR Primer 1 (100% over 44bp)
GGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG52600.10055771295819954Illumina Single End PCR Primer 1 (100% over 45bp)
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG52380.10013712936788007Illumina Single End PCR Primer 1 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)