FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10541437
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC429470.4074112476316085No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC363390.34472529694006615No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC139570.13240130354144317No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA59200.065.874914
GCGACAT59850.065.31485
CTGCGAC65850.059.5137523
GCTGCGA70950.055.370762
CGACATC82700.047.3257646
GGCTGCG87700.045.0734941
ACCCGAC31250.033.593983
TCGACCG15750.032.874953
GACATCT120950.032.6730167
GCTCGCG15800.032.4668779
ACATCTG125000.031.6145638
CTAGGCG176600.031.064417
TAGGCGG181300.030.2330428
CCCGACT35700.030.2034284
CCGACTC34700.029.8402845
TGGCTAG191300.029.2998644
GCTAGGC190950.028.8545466
AACCCGA37500.028.7555732
GGCTAGG197200.028.0845575
CATCTGT141900.027.7823879
Produced by FastQC (version 0.11.5)