FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10541437
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG181320.17200690949440764No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA108890.10329711214894137No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG24450.044.7277455
CGGGGAT136200.038.915888
TCGGGGA139900.037.9215287
CTGTCGG28400.037.672034
TCCGACC29850.035.8085639
GTCCCGT16650.033.9688951
CCCCGTT29150.033.1198732
TCTGTCG32150.032.6920783
GTCGGGG36100.031.6036516
TTCCGAC34200.031.1351538
GTAGCAA91150.031.1147569
TAGTAGC89500.030.9659847
CCGTTTA19850.030.9018234
ATCGGGG104900.030.6340486
GTTCCGT17100.030.2955651
TCAGTCG59800.030.0624884-85
TATCGGG88150.029.6127055
CTATCGG83600.029.5768534
CCGATCG55600.029.5143686-87
AGTCGGT61600.029.453986-87
Produced by FastQC (version 0.11.5)