FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8999836
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG117120.13013570469506333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA72600.047.0592965
TCAACGC75550.045.142744
ACGCAGA77450.044.7008367
CGCAGAG78800.043.4832958
ATCAACG79050.043.295323
AACGCAG84100.041.023856
GTATCAA91650.039.446121
GCAGAGT107450.032.2818459
GGTATCA43250.028.0926151
AGTACGG105550.023.45263912-13
GAGTACG92800.023.43667412-13
GCAGCGC15000.023.398448
TATCAAC154100.022.749822
ACGGGAC14800.021.7173613
TCTGTCG13850.020.6166278
GTACGGG116700.020.1082131
GCTCTAG24650.020.0543821
AGAGTAC131050.019.75085410-11
AATGCGT30400.019.5672996
AAATGCG34000.018.0313595
Produced by FastQC (version 0.11.5)