FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9518524
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG3375713.5464637164333466No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA1964872.0642591225278206No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1913702.0105007877271728No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1499771.5756329447716895No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1316761.383365740318562No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG919620.9661371868159392No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA878890.9233469390842529No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA751740.7897653039483853No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG743710.7813291220361476No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG725310.7619983938686292No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA705590.7412808960717019No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA640100.6724782119580724No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG606790.6374832904765487No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT529750.5565463721055912No Hit
TGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA507850.5335386032540339No Hit
GTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA467630.4912841528791649No Hit
TGTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA410800.43157951800090016No Hit
GGTGGCAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA398680.4188464514036No Hit
CTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA371700.3905017206449235No Hit
TGTGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG336520.3535422088550704No Hit
ATGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA322710.33903365689890574No Hit
GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTAC320440.33664883336954343No Hit
TGGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA319760.3359344368937873No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN311980.3277609007446953No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG304010.3193877538156126No Hit
TGGGGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG266700.2801905001237587No Hit
GTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG265630.27906637625749536No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC256070.26902280227480646No Hit
AGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA246500.25896872246159175No Hit
TGCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA243550.2558695024564733No Hit
AGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG242990.2552811759470271No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA216090.22702049183255724No Hit
GTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA215890.2268103752220407No Hit
TGGCTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA213710.22452010416741083No Hit
TGGTAATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG212640.22339598030114752No Hit
TGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTA207910.2184267224624322No Hit
GACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCCACTTCGGCCTT205920.21633606218779297No Hit
TTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG199640.20973840061757473No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA196040.20595630162827766No Hit
GGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA195550.2054415159325122No Hit
TGGTCGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG188290.19781428297076312No Hit
TGGTTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA186180.195597552729814No Hit
CGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG165150.17350379113400355No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG163270.17152869499514842No Hit
GCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG162360.1705726644172983No Hit
TTGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA162080.1702785011625752No Hit
TAGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG160790.16892324902474376No Hit
GGTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA156390.16430068359338065No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC154420.1622310349797931No Hit
TTGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA147360.15481391862856048No Hit
TGGCTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG143160.15040146980771388No Hit
TGAGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA141140.14827929204149717No Hit
GATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCA140690.14780652966783506No Hit
TGGTTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC137630.14459174552693255No Hit
TGGTTATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG137090.14402443067853796No Hit
TAGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA136910.14383532572907312No Hit
TGGCCGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG128270.13475828815476013No Hit
GGTTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG126640.13304583777905063No Hit
GTGGGTTTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAAC126580.13298280279589567No Hit
TGTTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG123680.12993611194340635No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT122080.12825517905927433No Hit
TGGATGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA121060.12718358434564014No Hit
TGGCAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG120130.1262065421067384No Hit
TGCTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG117770.12372716610264363No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA116080.12195168074377918No Hit
GGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG116030.12189915159115006No Hit
GGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG110140.11571121741143901No Hit
GATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGT109890.11544857164829338No Hit
TGATGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG108250.11372561544205805No Hit
TGATTCTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC107690.11313728893261182No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103100.10831511272125804No Hit
TGTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA102800.10799993780548328No Hit
TCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG101690.10683379061711669No Hit
GTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAAC98280.10325130240781029No Hit
TGTGGCAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA97770.10271550505099321No Hit
TTGTGGAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC96370.10124468877737768No Hit
CGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA96070.10092951386160291No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCTA159800.085.885372
GTGCTAG163450.085.674633
CGCGATA5600.085.672833
TGGTGTT233450.085.102091
TGCTAGT171850.084.499794
GGTGTTA261900.082.42092
TGATAGT223300.081.6655964
TGGTGCT163250.081.654961
GATAGTA275050.080.672225
GCTAGTA188000.078.6334845
GTGATAG222550.077.693283
GTGTTAG289450.077.443713
GGTGATA213350.077.256482
TGGTTAG72550.074.725241
TGGGGTA122550.074.1812441
TGGTGAT208850.073.668731
ATAGTAG368750.071.983316
TGGGGGT140900.068.967671
TAATAGT33100.068.310694
GGGGGTA151200.067.724562
Produced by FastQC (version 0.11.5)