FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18242936
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC760000.4165996087471885No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC360540.19763266176014652No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC258380.14163290382644547No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTTGGCTGATC230380.12628449718839116No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA77500.065.885394
GCGACAT80300.063.4105645
CTGCGAC87900.058.2007063
GCTGCGA93850.054.8649832
GGCTGCG125250.041.984751
CGACATC121600.041.7560466
ACCCGAC48900.032.7357643
CTAGGCG268700.030.8808027
TAGGCGG278700.029.6875618
TGGCTAG283950.029.6585034
GCTAGGC286600.029.1351936
GGCTAGG300650.027.9478875
CCCGACT57600.027.7076194
GACATCT189750.027.1338147
TTGATCG97950.026.40379122-23
TGATCGC97450.026.36928724-25
TCGACCG19300.026.334673
CCGACTC59500.026.1841965
ACATCTG197150.026.0430768
GGCTGGC334100.026.0193371
Produced by FastQC (version 0.11.5)