FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18242936
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG305080.16723185346920036No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT210100.040.654478
TGTCGGG42450.039.7357185
TCGGGGA218700.039.185817
TTCCGAC56150.037.395318
TCCGACC61400.033.811079
ATCGGGG160400.032.9330526
CCCCGTT59750.032.2952
TCTATCG119150.031.8713933
CTATCGG124050.031.5255054
CTGTCGG53250.031.2325464
CCGTTTA32300.030.3056554
GTCCCGT30100.030.1587961
TATCGGG137600.030.1112255
CCCGTTA25600.030.0762273
CCCCCGT41350.029.9993691
GTCGGGG58950.029.9771756
GCCCCGT40600.029.8511641
TCTGTCG55500.029.373083
GTAGCAA156000.029.2951879
TAGTAGC154400.029.1676987
Produced by FastQC (version 0.11.5)