FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6976610
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT645360.9250337914832562No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326130.4674619908522908No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251830.36096327586033905No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116570.1670868803043312No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG85090.12196467912066176No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100450.081.5325851
GTATCAA271550.068.758661
ATCAACG335750.055.1296543
TCAACGC339100.054.620134
CAACGCA340450.054.4559865
AACGCAG354850.052.348536
TATCAAC383000.048.56362
ACGCAGA389450.047.7116287
CGCAGAG390900.047.5194248
GCAGAGT424000.043.7396139
GCAGCGC12850.043.5239838
TCTGTCG11500.032.0772978
AGAGTAC439700.031.00077210-11
CAGAGTA445300.030.11654910-11
GCTCTAG17750.029.173171
GTGGTAT42200.028.9137291
TGGTATC40350.028.026552
GTCGTGC6250.026.6649481
AGTACTT435750.026.13415312-13
GAGTACT435000.025.789412-13
Produced by FastQC (version 0.11.5)