FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7028965
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA101770.14478660798567072No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG95710.13616513953334525No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA66050.046.2885
ATCAACG68500.044.8147283
TCAACGC69400.044.219074
GCAGCGC11300.043.7060668
GTATCAA78450.041.9901921
CGCAGAG74100.041.5158968
ACGCAGA74050.041.4603277
AACGCAG74850.040.8573386
GGTATCA35050.037.0496521
GCAGAGT92750.033.2375569
GCTCTAG19050.027.5171451
TCTGTCG12150.024.9767538
TATCAAC131050.024.1510642
GAGTACG86600.023.57514812-13
AGTACGG95700.022.23526812-13
CTAGCCT51250.021.7003694
GTCTCGC12050.021.2567621
TACGGGT16300.021.1621514
AGAGTAC109400.020.53724910-11
GACGCTA14350.019.69163328-29
Produced by FastQC (version 0.11.5)