FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004914073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15042412
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG6629764.407378284812303No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA3791292.520400318778664No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG3783342.5151152621002537No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG2955041.9644721870402166No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG2622831.7436232965830214No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1742601.158457832427406No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA1658071.1022633870153271No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1426460.948292069117639No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA1424650.9470888046411706No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1410240.9375092239196745No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT1352020.8988053245716179No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA1317550.875890116558435No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA1192260.7925989528806949No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1142570.7595656866731213No Hit
TGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA941610.6259700904349648No Hit
GTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA873480.5806781518814935No Hit
GGTGGCAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA789000.5245169458195933No Hit
TGTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA782060.5199033240147923No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG719820.4785269809123696No Hit
CTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA695950.46265851513706713No Hit
TGGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA647920.43072879535542574No Hit
TGTGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG633230.4209630742729291No Hit
ATGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA612660.4072884056094196No Hit
TGGGGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG502790.33424825752678494No Hit
GTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG500800.3329253313896734No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN493780.32825852662458654No Hit
GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTAC492640.3275006694405126No Hit
TGCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA463690.30825508568705606No Hit
AGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA457740.30429960301579295No Hit
AGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG456190.3032691831602538No Hit
GTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA408910.2718380536312926No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC403230.2680620634509944No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA401340.26680561601424024No Hit
TGGTAATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG397740.2644123828013752No Hit
TGGCTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA395110.26266399298197657No Hit
TTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG376930.250578165257008No Hit
GGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA375090.24935495717043252No Hit
GTGGGTTTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAAC368480.24496071507681083No Hit
TGGTTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA362720.24113154193622674No Hit
TGGTCGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG352340.23423105283913245No Hit
TGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTA334500.22237125269537888No Hit
GATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCA311170.20686177190200614No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC309850.20598425305728896No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA306180.20354448475417372No Hit
GCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG305800.20329186569281577No Hit
GGTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA301870.20067925276877138No Hit
TAGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG292560.19449008576550092No Hit
TTGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA291950.194084565693321No Hit
TTGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA280280.18632650136161674No Hit
GTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAAC272830.1813738381849932No Hit
TGGCTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG271670.1806026852608478No Hit
CGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG271410.18042984063991865No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG270290.1796852791959162No Hit
TGAGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA257350.17108293536967342No Hit
TAGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA255440.1698131921928478No Hit
GATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGT243290.1617360300994282No Hit
GTGGGGTTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAAC242060.16091834208503264No Hit
TGGTTATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG239250.15905029060499074No Hit
TGGCCGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG236300.15708916894444852No Hit
GGTTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG235970.15686978923326922No Hit
TGGCAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG235020.1562382415798743No Hit
TGTTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG227880.1514916623743586No Hit
GGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG226100.15030834150799752No Hit
TGCTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG219340.14581438136383978No Hit
TGGATGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA217300.14445821587654958No Hit
TGGTTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC209740.13943242612953294No Hit
GTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCA208930.13889394865663832No Hit
GACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCCACTTCGGCCTT204950.13624809638241528No Hit
TGTGGCAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA204830.13616832194198641No Hit
GGGGCGAAGCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGA204390.13587581566041404No Hit
TGATGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG198450.13192698085918667No Hit
TGTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA198400.131893741509008No Hit
TGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTG192480.1279582024478521No Hit
TTGTGGAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC191230.12712721869338506No Hit
GTGGGCTTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAAC184250.12248700540844115No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA183250.12182221840486751No Hit
TCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG179230.11914977465050153No Hit
TGGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA177500.11799969313431916No Hit
GGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA172980.11499485587816635No Hit
GGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA172600.11474223681680837No Hit
CGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA168240.11184376548122735No Hit
GGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA167860.11159114641986936No Hit
TTGTGTAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC167600.11141830179894022No Hit
TGATTCTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC166900.11095295089643868No Hit
GGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGTAA166800.11088647219608132No Hit
GTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT161180.10715036923599752No Hit
GGCTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG158690.1054950495970992No Hit
TGGCGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG153070.1017589466370154No Hit
TTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGC152840.10160604562619345No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)