FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9639011
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC203510.2111316192086512No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC162690.16878287616851978No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT132770.1377423472179874No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG114050.11832126760722651No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT110390.11452419755512261No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC108290.11234555080391545No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA31350.050.3014074
GCGACAT32900.048.2178275
CTGCGAC38600.041.839333
CGACATC41450.038.0430346
GCTGCGA43900.036.249182
TCGACCG9600.030.1822573
CTAGGCG101350.027.4620637
GGCTGCG60500.027.0909631
TAGGCGG104600.026.745028
GCTAGGC110950.025.3000726
TGGCTAG117000.024.723564
GCGCGGT50950.024.5173286
GGCTAGG118850.024.097685
CCGCTCG12100.023.9443917
ACTCGGG120000.023.6744921
GCTCGCG12550.023.0858259
CTCGGGA126050.022.7620812
TCGGGAG129000.022.2402843
GGGCGCG57700.021.8970414
CGACCGC13700.021.8423614
Produced by FastQC (version 0.11.5)