FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9639011
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG117380.1217759788841407No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG100730.1045024224995697No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC57000.053.920799
TTCCGAC59250.051.988958
TTTCCGA79200.039.3740237
CCGTTTC66350.038.4168664
GTTTCCG68500.037.5441556
CGTTTCC69400.037.2718625
GCTCCGT48650.037.221541
CCCCGTT27250.037.001692
CGGGGAT80650.036.9562238
CTCCGTT57600.035.257992
TCACCCC181200.035.1558349
GTTCCGT16300.034.9901581
TCCGTTT73300.034.973333
ACCGGTT10600.034.1004373
TCGGGGA87950.033.997427
CCGTTAC7500.033.605684
TTCACCC193900.032.7041138
CCCGTTT36350.032.448863
GTTCACC202150.031.346397
ATAGCGC113150.031.16695294-95
Produced by FastQC (version 0.11.5)