FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7017760
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT654140.9321207906796471No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334360.476448325391578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256800.3659287293951346No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117770.167817081233898No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG83280.11867034495337543No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99050.079.370431
GTATCAA264900.070.0269551
ATCAACG332100.055.5870973
TCAACGC336850.054.8028644
CAACGCA337850.054.6230355
AACGCAG350450.052.7523736
TATCAAC381400.048.5429952
ACGCAGA387350.047.6635637
CGCAGAG389000.047.4613888
GCAGAGT425250.043.4015969
GCAGCGC12350.042.877688
AGAGTAC436250.031.35535810-11
CAGAGTA438750.029.95620510-11
CGTCAAT9750.029.292976
GCTCTAG18700.027.369521
GTGGTAT40650.026.9381431
TGGTATC38300.026.260032
AGTACTT434700.026.25228912-13
GAGTACG84200.025.72157512-13
GTCGTGC7650.025.6721881
Produced by FastQC (version 0.11.5)