FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9031435
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC260470.28840378079452494No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC257720.28535886046901743No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG156240.17299576423901628No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC123220.13643457545783144No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA46350.055.9527634
GCGACAT47300.055.0273785
CTGCGAC52450.049.6306343
GCTGCGA55150.047.4605562
CGACATC56250.045.7655376
GGCTGCG69050.038.3246651
TCGACCG9650.034.9498863
CCGCTCG10000.034.1969267
GCTCGCG10900.030.5020169
CGACCGC12600.027.141924
GCGCGGT62950.026.4079176
GACATCT99300.026.163317
CTCGCGT6500.025.57483710-11
CTAGGCG129650.025.4238647
CTCGACC13800.025.1287272
ACATCTG104800.024.8810168
TAGGCGG133000.024.7479138
TCGCGTC7050.024.59019510-11
CGCGTCG6900.024.09237112-13
CGCGGTG69250.024.0736487
Produced by FastQC (version 0.11.5)