FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9031435
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG141210.1563538906054243No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG108240.11984806401197595No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC54300.052.9295049
TTCCGAC55950.051.8229648
TTTCCGA67850.042.5244377
TGTCGGG21100.042.136885
GTTTCCG62100.040.1848756
CGGGGAT102100.039.571798
TCGGGGA104100.038.9491547
CGTTTCC62850.038.4292265
CCGTTTC65200.038.3596884
GTTCCGT15900.036.1702771
CTCCGTT49700.035.7871252
CTGTCGG24000.035.661384
GCTCCGT44700.035.4079131
CCCCGTT27300.035.0142632
TCCGTTT65250.034.619813
CCCGTTT34200.034.3464933
TCACCCC156400.032.7130669
GTTCACC161800.031.4931417
TTCACCC164350.031.177528
TCTGTCG27800.030.4499633
Produced by FastQC (version 0.11.5)