FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9993287
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA167240.16735234362827767No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG144740.14483722923198342No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG104020.10408987553344559No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC50250.056.153999
TTCCGAC52550.053.5521778
TTTCCGA57200.049.9476787
GTTTCCG52000.048.725516
TCGACGT5350.047.1185342
TTCGACG4550.047.009041
TCGCTCG5700.045.059733
GCTCCGT39800.044.784581
CTCCGTT43050.042.7570232
CGTTTCC57150.042.596655
TCCGTTT58250.040.255083
TCGATCG22900.040.0646
GCCCCGT22750.039.6965261
CTCGCCG40750.039.662846
CCGTTTA21000.038.720984
TAGTAGC86100.038.372367
CCCGTTA14400.038.314683
GTTCCGT16800.037.6289981
TGTCGGG16950.036.4666065
GTAGCAA92100.036.2598469
Produced by FastQC (version 0.11.5)